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Antibacterial activities of some medicinal plant extracts?

Antimicrobial activity of 18 ethnomedicinal plant extracts was

evaluated against nine bacterial strains and one fungal strain.

The collected ethno medicinal Plant extracts were used in folk

medicine in the treatment of skin diseases, venereal diseases,

respiratory problems and nervous disorders.

According to World Health Organization more than 80% of the

world's population relies on traditional medicine for their

primary healthcare needs. Plant extracts used for traditional

medicine contain a wide range of substances that can be used to

treat chronic as well as infectious diseases.

Plant extracts were collected from Palni hills of Southern

Western Ghats and the ethnobotanical data were gathered from

traditional healers who inhabit the study area. The hexane and

methanol extracts were obtained by cold percolation method and

the antimicrobial activity was found using paper disc diffusion

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This plant has a history of use for the treatment of various

ailments and the most commonly used plant part for this purpose

is the root, but flowers bark and fruit can also be utilized.

A sensitivity test performed with commonly used sensitivity test

disks resulted in the appearance of multiple drug resistance

phenotypes of the bacteria tested. A comparison of data in the

inhibition zones of pathogenic bacteria showed that gentamycin,

ofloxacin, kanamycin and tobramycin were effective against all of

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Screening of medicinal plant extracts for antimicrobial

activities and phytochemicals is important for finding potential

new compounds for therapeutic use. This paper reports the results

of a survey that was done based on folk uses by traditional

practitioners in Palni hills of Tamil Nadu along with bioassay

test for antimicrobial activity.

Plant extracts were prepared by cold percolation method. The

plant materials were dried under shade and ground into fine

powder using electric blender. 50 g of dried powder was soaked in

300 ml hexane for 48 hours with intermittent shaking. The plant

extracts were filtered through Whatman No. 1 filter paper into

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The processing of the plants performed in this study was not

comparable to the traditional approach when the Paliyar tribe

used water for extracts whereas we have used hexane and methanol

for extraction. In this sense it is not an exact replication of

the traditional knowledge.

Antibacterial and Antifungal activities of the plant extract were

tested using Well diffusion method. The prepared culture plates

were inoculated with different selected strains of bacteria and

fungi using streak plate method. Wells were made on the agar

surface with 6mm cork borer. The extracts were poured into the

well using sterile syringe.

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